Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic, membrane and nuclear staining of glandular cells.Application
Western blot analysis of ADAM12 precursor expression in F9 (A), JAR (B), Sol8 (C) and BT-20 (D) whole cell lysates.| Product Name | ADAM12 Antibody (H-210) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 700-909 mapping at the C-terminus of ADAM12 of human origin |
| Modification Notes | ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNFå (tumor necrosis factor å), and modulation of the neurogenic function of Notch and Delta (1-3). ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail (1-3). ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone, and muscle, and catalyze proteolysis, adhesion, fusion, and intracellular signaling (3). ADAM 12 (Meltrin-a) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion (4-6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ADAM12 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 700-909 mapping at the C-terminus of ADAM12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 700-909 mapping at the C-terminus of ADAM12 of human origin |
| Entrez Gene | 8038 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic, membrane and nuclear staining of glandular cells.
Application
Western blot analysis of ADAM12 precursor expression in F9 (A), JAR (B), Sol8 (C) and BT-20 (D) whole cell lysates.| Application Notes | recommended for detection of ADAM12 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
