Application
Western blot analysis of CB1 expression in U-87 MG (A), RAW 264.7 (B) and IMR-32 (C) whole cell lysates and rat testis tissue extract (D).| Product Name | CB1 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-150 mapping at the N-terminus of CB1 of human origin |
| Modification Notes | The cannabinoid receptors (CB1 and CB2) are G-protein coupled receptors that inhibit adenylate cyclase activity in response to psychoactive cannabinoids (1-3). CB1 is expressed in brain tissue and, in low levels, in testis (1,4). CB2 has been shown to be expressed only by cells of the immune system, specifically by HL-60 cells (5). The cannabinoid receptors mediate most of the cannabinoid-induced responses in a dose-dependent, stereoselective manner (1,3). Phosporylation of CB1 on serine 316 leads to a disruption of CB1-mediated inhibition of calcium channels and activation of potassium currents (6). This response system is thought to be involved in specific brain functions, such as nociception, control of movement, memory, and neuroendocrine regulation as well as having a possible role in brain development (7). In addition, CB1 may mediate the addictive behavior involved with the use of psychoactive cannabinoids, such as THC in marijuana (3,8,9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CB1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-150 mapping at the N-terminus of CB1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-150 mapping at the N-terminus of CB1 of human origin |
| Entrez Gene | 1268 |
|---|
Application
Western blot analysis of CB1 expression in U-87 MG (A), RAW 264.7 (B) and IMR-32 (C) whole cell lysates and rat testis tissue extract (D).| Application Notes | recommended for detection of CB1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
