Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing golgi apparatus localization.Application
Western blot analysis of golgin 84 expression in K-562 (A) and SW-13 (B) whole cell lysates.| Product Name | golgin 84 Antibody (H-283) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 343-625 mapping within an internal region of golgin 84 of human origin |
| Modification Notes | Golgin 84, also known as GOLGA5 (Golgi autoantigen, subfamily A, member 5), RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein. Localizing to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack, golgin 84 participates in the maintenance of Golgi structure and the formation of Golgi stacks and ribbons. The depletion of golgin 84 leads to fragmentation of the Golgi ribbon and reduced efficiency in protein transport. In addition, golgin 84 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport. During mitosis, golgin 84 is highly phosphorylated. Chromosomal translocations involving the gene encoding golgin 84 have been associated with cancer tissues. The chimeric proteins produced by this translocation are known as Ret-II and PTC5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of golgin 84 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 343-625 mapping within an internal region of golgin 84 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 343-625 mapping within an internal region of golgin 84 of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing golgi apparatus localization.
Application
Western blot analysis of golgin 84 expression in K-562 (A) and SW-13 (B) whole cell lysates.| Application Notes | recommended for detection of golgin 84 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
