Application
Western blot analysis of HDA1 expression in 5 µg C. elegans (A) and 10 µg C. elegans (B) extract.| Product Name | HDA1 Antibody (Ce-87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 374-460 mapping at the cC-terminus (Ce) |
| Modification Notes | Remodeling of chromatin structure is believed to be a critical component of transcriptional regulation. A major source of remodeling is brought about by the acetylation of nucleosomal histones. Acetylation results in an allosteric change in the nucleosomal conformation and an increased accessibility of DNA to transcription factors. Conversely, the deacetylation of histones is associated with transcriptional silencing. HDA1, HDA2, HDA3, HDA4, HDA5, HDA6 and HDA7 have been identified as histone deacetylases in C. elegans and are homologous with histone deacetylase proteins in both yeast and mammalian systems (2,3). Histone deacetylase HDA1 (also known as Hda1 or N2819 in yeast), deacetylates Histone H2A, H2B, H3 and H4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HDA1 (also designated RPD3-1) by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 374-460 mapping at the cC-terminus (Ce) |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 374-460 mapping at the cC-terminus (Ce) |
Application
Western blot analysis of HDA1 expression in 5 µg C. elegans (A) and 10 µg C. elegans (B) extract.| Application Notes | recommended for detection of HDA1 (also designated RPD3-1) by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
