Application
Western blot analysis of β1-AR expression in Hep G2 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic and membrane staining.Application
Western blot analysis of β1-AR expression in SK-N-MC (A), Hep G2 (B) and A-431 (C) whole cell lysates.| Product Name | β1-AR Antibody (V-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of beta1-AR of mouse origin |
| Modification Notes | Beta1 adrenergic receptors (AR) bind cathecholamines (epinephrine, norepinephrine), and influence development, behavior, cardiac function, smooth muscle tone, and metabolism. Beta1-ARs are present in the heart, juxtaglomerular cells, and in the central and peripheral nervous systems. Beta1-adrenoceptor and beta2-adrenoceptor couple to Gs-proteins to activate adenylyl cyclase. The agonist-specific component of beta1AR downregulation requires internalization correlating with receptor degradation, while the cAMP-specific component does not require internalization and is associated with downregulation of beta1AR mRNA. Internalization of beta(1)AR is both arrestin- and dynamin-dependent and follows the same clathrin-mediated endocytic pathway as beta(2)AR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of beta1 adrenergic receptor of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of beta1-AR of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of beta1-AR of mouse origin |
| Entrez Gene | 153 |
|---|
Application
Western blot analysis of β1-AR expression in Hep G2 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic and membrane staining.
Application
Western blot analysis of β1-AR expression in SK-N-MC (A), Hep G2 (B) and A-431 (C) whole cell lysates.| Application Notes | recommended for detection of beta1 adrenergic receptor of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
