Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of CNTF expression in non-transfected: (A) and human CNTF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant CNTF.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.| Product Name | CNTF Antibody (FL-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-200 representing full length CNTF of human origin |
| Modification Notes | Ciliary neurotrophic factor, or CNTF, is a neuropoietic cytokine that promotes the survival and differentiation of a number of cell types including sensory, sympathetic and motor neurons. CNTF, LIF and IL-6 belong to a family of cytokines that share structural homology and signal through identical receptor components. The CNTF receptor (CNTFR) is comprised of CNTFRå, a CNTFR-specific chain, and a heterodimer of the gp130 chain common to the IL-6 and LIF receptor and the LIFRb chain. The CNTFR complex has been shown to augment DNA synthesis through the activation of transcription factors Stat1 and Stat3. CNTF has been implicated as a protein involved in the pathogenesis of amyotrophic lateral sclerosis, or ALS. However, unlike mice lacking CNTF, mice containing a homozygous null mutation in the gene encoding the CNTFRå chain die perinatally and display severe motor neuron deficits. This data suggests the existence of a second CNTFR ligand that plays a critical role in development of the neonatal nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CNTF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-200 representing full length CNTF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-200 representing full length CNTF of human origin |
| Entrez Gene | 1270 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of CNTF expression in non-transfected: (A) and human CNTF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant CNTF.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.| Application Notes | recommended for detection of CNTF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
