Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma showing cytoplasmic localization.Application
Western blot analysis of human recombinant Ob protein (leptin).Application
Immunofluorescence staining of methanol-fixed JAR cells showing cytoplasmic localization.| Product Name | Ob Antibody (A-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Ob of human origin |
| Modification Notes | Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob (also designated leptin) results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It have been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The gene encoding Ob maps to human chromosome 7q31.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ob of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine, avian and feline: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Ob of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Ob of human origin |
| Entrez Gene | 3952 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma showing cytoplasmic localization.
Application
Western blot analysis of human recombinant Ob protein (leptin).
Application
Immunofluorescence staining of methanol-fixed JAR cells showing cytoplasmic localization.| Application Notes | recommended for detection of Ob of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine, avian and feline: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
