Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.Application
Western blot analysis of RyR expression in rat heart tissue extract.Application
Western blot analysis of human recombinant RyR fusion protein.| Product Name | RyR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RyR of human origin |
| Modification Notes | Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The å1 subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR å1 subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR a1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a several-fold reduction in Ca2+ current density. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of skeletal muscle, cardiac muscle and brain ryanodine receptors of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RyR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RyR of human origin |
| Entrez Gene | 6261 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.
Application
Western blot analysis of RyR expression in rat heart tissue extract.
Application
Western blot analysis of human recombinant RyR fusion protein.| Application Notes | recommended for detection of skeletal muscle, cardiac muscle and brain ryanodine receptors of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
