Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of GRK 3 expression in non-transfected: (A) and human GRK 3 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of GRK 3 expression in non-transfected: (A) and mouse GRK 3 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of GRK 3 expression in non-transfected control (A) and GRK 3 siRNA transfected (B) HeLa cells. Blot probed with GRK 3 (C-14): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of GRK 3 expression in BJAB (A), Ramos (B), HL-60 (C) and MOLT-4 (D) whole cell lysates.| Product Name | GRK 3 Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of GRK 3 of bovine origin |
| Modification Notes | Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of ∫-adrenergic receptor kinase: GRK 2 (∫ARK, ∫ARK1) and GRK 3 (∫ARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GRK 3 of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of GRK 3 of bovine origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of GRK 3 of bovine origin |
| Entrez Gene | 157 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of GRK 3 expression in non-transfected: (A) and human GRK 3 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of GRK 3 expression in non-transfected: (A) and mouse GRK 3 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of GRK 3 expression in non-transfected control (A) and GRK 3 siRNA transfected (B) HeLa cells. Blot probed with GRK 3 (C-14): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of GRK 3 expression in BJAB (A), Ramos (B), HL-60 (C) and MOLT-4 (D) whole cell lysates.| Application Notes | recommended for detection of GRK 3 of mouse, rat, human and bovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
