Application
Western blot analysis of human recombinant NEIL1 fusion protein.Application
Western blot analysis of NEIL1 expression in non-transfected: (A) and human NEIL1 transfected: (B) 293 whole cell lysates.| Product Name | NEIL1 Antibody (H-49) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-49 mapping at the N-terminus of NEIL1 of human origin |
| Modification Notes | NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1,2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG familiy of proteins. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2. NEIL1 is a ubiquitiously expressed protein that is up-regulated during S-phase. NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEIL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-49 mapping at the N-terminus of NEIL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-49 mapping at the N-terminus of NEIL1 of human origin |
Application
Western blot analysis of human recombinant NEIL1 fusion protein.
Application
Western blot analysis of NEIL1 expression in non-transfected: (A) and human NEIL1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of NEIL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
