Application
Western blot analysis of IFN-α/βRα expression in non-transfected: (A) and human IFN-α/βRα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 20 ng IFN-α/βRα (D-457) fusion protein.| Product Name | IFN-α/βRα Antibody (R-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 458-557 mapping at the C-terminus (complete intracellular domain) of the IFN-α/βR alpha chain precursor of human origin |
| Modification Notes | The type I interferons (IFNs), å and ∫, are a group of structurally and functionally related proteins that are induced by either viruses or double stranded RNA and defined by their ability to confer an antiviral state in cells. The å and ∫ IFNs appear to compete with one another for binding to a common cell surface receptor while immune IFN (IFN©) binds to a distinct receptor. The latter protein, IFN-åR, is only weakly responsive to type I interferons in contrast to IFN-å/∫R, which binds to and responds effectively to IFN-∫ and to several of the IFN-å subtypes. Moreover, IFN-å/∫R is physically associated with the cytoplasmic tyrosine kinase JAK1 and thus, in addition to ligand binding, appears to be functionally involved in signal transduction. The IFN-© receptor complex consists of an alpha subunit (IFN-©Rå) and a beta subunit that is 332 amino acids in length (mouse) and 337 amino acids in length (human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IFN-α/βR alpha chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 458-557 mapping at the C-terminus (complete intracellular domain) of the IFN-&alpha /&beta R alpha chain precursor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 458-557 mapping at the C-terminus (complete intracellular domain) of the IFN-α/βR alpha chain precursor of human origin |
| Entrez Gene | 3454 |
|---|
Application
Western blot analysis of IFN-α/βRα expression in non-transfected: (A) and human IFN-α/βRα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 20 ng IFN-α/βRα (D-457) fusion protein.| Application Notes | recommended for detection of IFN-α/βR alpha chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
