Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG:. Antibody tested:E2F-4 (A-20): .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated chicken anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of E2F-4 expression in non-transfected: (A) and mouse E2F-4 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.Application
Western blot analysis of E2F-4 expression in Jurkat (A) and Y79 (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.| Product Name | E2F-4 Antibody (A-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of E2F-4 of human origin |
| Modification Notes | The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of E2F-4 and, to a lesser extent, E2F-5 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of E2F-4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of E2F-4 of human origin |
| Entrez Gene | 1874 |
|---|
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG:. Antibody tested:E2F-4 (A-20): .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated mouse anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated chicken anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of E2F-4 expression in non-transfected: (A) and mouse E2F-4 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
Application
Western blot analysis of E2F-4 expression in Jurkat (A) and Y79 (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.| Application Notes | recommended for detection of E2F-4 and, to a lesser extent, E2F-5 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
