Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of cyclin T1 expression in A-431 (A) and K-562 (B) nuclear extracts.| Product Name | cyclin T1 Antibody (H-245) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-505 mapping to an internal region of cyclin T1 of human origin |
| Modification Notes | Cyclin T1 was identified as a partner for Cdk9, an RNA polymerase II (RNAPII) transcription elongation factor. Cyclin T1 interacts with the transactivation domain of the HIV-1 Tat protein. The interaction of Tat with cyclin T1 enhances the affinity of Tat for the viral TAR RNA stem-loop structure, suggesting that Tat can recruit cyclin T1/cdk9 to RNAPII through cooperative binding to TAR. The human positive transcription elongation factor b (P-TEFb) consists of a cyclin dependent kinase, cdk9, paired with a cyclin T. Cdk9 may be paired with either cyclin T1 or cyclin T2, in a mutually exclusive manner. Two forms of cyclin T2, T2a and T2b, are due to alternative splicing. The binding of Tat to TAR was shown to be facilitated by human cyclin T1, but not by cyclins T2a or T2b. Cyclin T2 binds to Cdk9 but not to Tat, and cyclin T2 can inhibit cyclin T1-mediated Tat activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of cyclin T1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-505 mapping to an internal region of cyclin T1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-505 mapping to an internal region of cyclin T1 of human origin |
| Entrez Gene | 904 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of cyclin T1 expression in A-431 (A) and K-562 (B) nuclear extracts.| Application Notes | recommended for detection of cyclin T1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
