Application
Western blot analysis of PREP-1 expression in A-431 nuclear extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.| Product Name | PREP-1 Antibody (N-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 15-436 mapping at the N-terminus of PREP-1 (Pbx-regulating protein-1) of human origin |
| Modification Notes | Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) chromosomal rearrangement which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Fusion cDNAs have been shown to encode a protein comprised of two-thirds of the E2A transactivation domain, fused to a homeobox protein termed Prl or Pbx 1. Two highly related Pbx proteins, designated Pbx 2 and Pbx 3, have also been identified. Pbx 2 and Pbx 3 share a 92% and 94% identity, respectively, with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Pbx-regulating protein-1, PREP-1 is a DNA-binding protein that forms stable complexes with Pbx proteins which synergize with AP-1 binding factors to augment transcription of the urokinase gene. Also referred to as UEF3, PRP-1 or p64, PREP-1 appears to be a general DNA-binding factor involved in modulating the transcriptional activity of AP-1 containing promoters. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of PREP-1 of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 15-436 mapping at the N-terminus of PREP-1 (Pbx-regulating protein-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 15-436 mapping at the N-terminus of PREP-1 (Pbx-regulating protein-1) of human origin |
| Entrez Gene | 5316 |
|---|
Application
Western blot analysis of PREP-1 expression in A-431 nuclear extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of PREP-1 of human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
