Application
Western blot analysis of human recombinant AQP4 fusion protein.Application
Western blot analysis of AQP4 expression in non-transfected: (A) and mouse AQP4 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing membrane staining of alveolar cells and macrophages. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.Application
Western blot analysis of AQP4 expression in non-transfected control (A) and AQP4 siRNA transfected (B) HeLa cells. Blot probed with AQP4 (H-80): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of AQP4 expression in mouse kidney tissue extract.| Product Name | AQP4 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 244-323 mapping at the C-terminus of aquaporin 4 (AQP4) of human origin |
| Modification Notes | In skeletal muscle, AQP4 (Aquaporin 4, also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AQP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 244-323 mapping at the C-terminus of aquaporin 4 (AQP4) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 244-323 mapping at the C-terminus of aquaporin 4 (AQP4) of human origin |
| Entrez Gene | 361 |
|---|
Application
Western blot analysis of human recombinant AQP4 fusion protein.
Application
Western blot analysis of AQP4 expression in non-transfected: (A) and mouse AQP4 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing membrane staining of alveolar cells and macrophages. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.
Application
Western blot analysis of AQP4 expression in non-transfected control (A) and AQP4 siRNA transfected (B) HeLa cells. Blot probed with AQP4 (H-80): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of AQP4 expression in mouse kidney tissue extract.| Application Notes | recommended for detection of AQP4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
