Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining in cells at granular and molecular layers, and cytoplasmic and nuclear staining in Purkinje cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tumor showing cytoplasmic staining.Application
Western blot analysis of GDNF expression in mouse brain extract.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.| Product Name | GDNF Antibody (D-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of GDNF of human origin |
| Modification Notes | Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated, disulfide-bonded homodimer and is a distantly related member of the TGF ∫ superfamily of growth regulatory ligands. GDNF contains the seven conserved cysteine residues in the same relative spacing characteristic of all members of the TGF ∫ superfamily. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings, it has been suggested that GDNF may have utility in the treatment of Parkinson’s disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GDNF p20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of GDNF of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of GDNF of human origin |
| Entrez Gene | 2668 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining in cells at granular and molecular layers, and cytoplasmic and nuclear staining in Purkinje cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tumor showing cytoplasmic staining.
Application
Western blot analysis of GDNF expression in mouse brain extract.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.| Application Notes | recommended for detection of GDNF p20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
