Application
Western blot analysis of caspase-12 expression in non-transfected: (A) and mouse caspase-12 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of caspase-12 expression in BC3H1 (A) and UV-treated NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.| Product Name | caspase-12 Antibody (M-108) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 38-145 mapping near the N-terminus of caspase-12 of mouse origin |
| Modification Notes | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is composed of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, caspase-10, caspase-14, and caspase-5/caspase-12. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-5 (also designated TY or ICErelIII) can cleave its own precursor, an activity that requires the cysteine 245 residue. The mouse homolog of caspase-5 is designated caspase-12. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating that caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of caspase-12 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 38-145 mapping near the N-terminus of caspase-12 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 38-145 mapping near the N-terminus of caspase-12 of mouse origin |
| Entrez Gene | 12364 |
|---|
Application
Western blot analysis of caspase-12 expression in non-transfected: (A) and mouse caspase-12 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of caspase-12 expression in BC3H1 (A) and UV-treated NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.| Application Notes | recommended for detection of caspase-12 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
