Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of Gα 11 expression in non-transfected 293T: (A), mouse Gα 11 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Product Name | Gα 11 Antibody (D-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the N-terminus of Gα 11 of mouse origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Ga subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gq class includes Gå 15, Gå 14, Gå 11 and Gå q, two of which, Gå 11 and Gå q, are abundant in brain and lung and present at lower levels in a variety of tissues (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gα 11 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA non cross-reactive with Gα q also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the N-terminus of G&alpha 11 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within the N-terminus of Gα 11 of mouse origin |
| Entrez Gene | 2767 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of Gα 11 expression in non-transfected 293T: (A), mouse Gα 11 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of Gα 11 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA non cross-reactive with Gα q also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
