Application
Western blot analysis of ATF-6α expression in MCF7 nuclear extract.| Product Name | ATF-6α Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-310 mapping at the N-terminus of ATF-6α of human origin |
| Modification Notes | ATF-6 is a member of the basic-leucine zipper family of transcription factors. Endoplasmic reticulum stress causes cleavage of transmembrane ATF-6 and transclocation of active ATF-6 to the nucleus. Soluble ATF-6 can exist as either an ATF-6∫ homodimer or an ATF-6å/∫ heterodimer. Binding of the ATF-6∫ homodimer or ATF-6å/∫ heterodimer to the nuclear transcription factor Y C (NF-YC) induces ER chaperone transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ATF-6α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-310 mapping at the N-terminus of ATF-6&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-310 mapping at the N-terminus of ATF-6α of human origin |
| Entrez Gene | 22926 |
|---|
Application
Western blot analysis of ATF-6α expression in MCF7 nuclear extract.| Application Notes | recommended for detection of ATF-6α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
