Application
Western blot analysis of Stat5b expression in SK-BR-3 (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of Stat5 expression in 293T (A) and Jurkat (B) whole cell lysates.Application
Western blot analysis of Stat5b expression in non-transfected 293T: (A), human Stat5b transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5 (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Stat5 expression in Y79 (A), A-673 (B), K-562 (C) and Jurkat (D) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.| Product Name | Stat5 Antibody (C-17) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Stat5 of mouse origin |
| Modification Notes | Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, FCM, ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Stat5 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Stat5 of mouse origin |
| Entrez Gene | 6776 |
|---|
Application
Western blot analysis of Stat5b expression in SK-BR-3 (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of Stat5 expression in 293T (A) and Jurkat (B) whole cell lysates.
Application
Western blot analysis of Stat5b expression in non-transfected 293T: (A), human Stat5b transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5 (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Stat5 expression in Y79 (A), A-673 (B), K-562 (C) and Jurkat (D) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.| Application Notes | recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, FCM, ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
