Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of IKKα expression in 293T (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization of IKKα.Application
Western blot analysis of IKKα expression in A-673 (A), BJAB (B) and Jurkat (C) whole cell lysates.| Product Name | IKKα Antibody (H-744) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-745 representing full length IKKα of human origin |
| Modification Notes | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IKKα and, to a lesser extent, IKKβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-745 representing full length IKK&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-745 representing full length IKKα of human origin |
| Entrez Gene | 1147 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of IKKα expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization of IKKα.
Application
Western blot analysis of IKKα expression in A-673 (A), BJAB (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of IKKα and, to a lesser extent, IKKβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
