Application
Western blot analysis of Heme Oxygenase 1 expression in non-transfected: (A) and mouse Heme Oxygenase 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Heme Oxygenase 1 expression in rat spleen tissue extract.Application
Western blot analysis of human recombinant Heme Oxygenase 1 fusion protein.| Product Name | Heme Oxygenase 1 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 184-288 mapping at the C-terminus of Heme Oxygenase 1 of human origin |
| Modification Notes | Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Heme Oxygenase 1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 184-288 mapping at the C-terminus of Heme Oxygenase 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 184-288 mapping at the C-terminus of Heme Oxygenase 1 of human origin |
| Entrez Gene | 3162 |
|---|
Application
Western blot analysis of Heme Oxygenase 1 expression in non-transfected: (A) and mouse Heme Oxygenase 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Heme Oxygenase 1 expression in rat spleen tissue extract.
Application
Western blot analysis of human recombinant Heme Oxygenase 1 fusion protein.| Application Notes | recommended for detection of Heme Oxygenase 1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
