Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Met expression in LADMAC whole cell lysate (A) and rat pancreas tissue extract (B).Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Product Name | Met Antibody (SP260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a C-terminal cytoplasmic domain of Met of mouse origin |
| Modification Notes | The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Met of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of Met of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of Met of mouse origin |
| Entrez Gene | 4233 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Met expression in LADMAC whole cell lysate (A) and rat pancreas tissue extract (B).
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Application Notes | recommended for detection of Met of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
