Application
Western blot analysis of Snk expression in non-transfected: (A) and human Snk transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Immunofluorescence staining of methanol-fixed Jar cells showing membrane localization.| Product Name | Snk Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-90 mapping at the N-terminus of Snk of human origin |
| Modification Notes | Plks (polo-like kinases) encode serine/threonine kinases that are closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively (1-4). Polo-like kinases, which include Plk, Snk (for serum-inducible kinase, also designated Plk2) and Fnk (for FGF-inducible kinase, also designated Plk3 or PRK), play a role in cell proliferation (5). Plk protein accumulates in the cell during S and G2 phases of the cell cycle, and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis (2). Snk and Fnk are immediate-early response genes that are first expressed during G1 phase (5,6). Fnk expression peaks in late S and G2 phases, and it may play a role in regulating the onset of M phase (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Snk of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-90 mapping at the N-terminus of Snk of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-90 mapping at the N-terminus of Snk of human origin |
| Entrez Gene | 10769 |
|---|
Application
Western blot analysis of Snk expression in non-transfected: (A) and human Snk transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Immunofluorescence staining of methanol-fixed Jar cells showing membrane localization.| Application Notes | recommended for detection of Snk of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
