Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells.Application
Antibodies tested include p-caveolin-1 (B-9): (A,B,C) and caveolin-1 (N-20): (D,E,F).Application
Antibodies tested include p-caveolin-1 (G-10): (A,B,C) and caveolin-1 (N-20): (D,E,F).Application
Antibodies tested include p-caveolin-1 (Tyr 14)-R: (A,B,C) and caveolin-1 (N-20): (D,E,F).Application
Antibodies tested include p-caveolin-1 (Tyr 14): (A,B,C) and caveolin-1 (N-20): (D,E,F).Application
Western blot analysis of caveolin-1 expression in non-transfected: (A) and human caveolin-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of caveolin-1 expression in non-transfected 293T: (A), mouse caveolin-1 transfected 293T: (B) and U-87 MG (C) whole cell lysates.Application
Western blot analysis of caveolin-1 expression in non-transfected: (A) and human caveolin-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of caveolin-1 expression in non-transfected 293: (A), human caveolin-1 transfected 293: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of caveolin-1 expression in non-transfected control (A) and caveolin-1 siRNA transfected (B) HeLa cells. Blot probed with caveolin-1 (N-20): . Actin (I-19): used as specificity and loading control.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing membrane and cytoplasmic staining.Application
Western blot analysis of caveolin-1 p22 expression in A-431 (A), NIH/3T3 (B), MDA-MB-231 (C) and SK-LMS-1 (D) whole cell lysates.| Product Name | caveolin-1 Antibody (N-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of caveolin-1 of human origin |
| Modification Notes | Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of α-caveolin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of caveolin-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of caveolin-1 of human origin |
| Entrez Gene | 857 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells.
Application
Antibodies tested include p-caveolin-1 (B-9): (A,B,C) and caveolin-1 (N-20): (D,E,F).
Application
Antibodies tested include p-caveolin-1 (G-10): (A,B,C) and caveolin-1 (N-20): (D,E,F).
Application
Antibodies tested include p-caveolin-1 (Tyr 14)-R: (A,B,C) and caveolin-1 (N-20): (D,E,F).
Application
Antibodies tested include p-caveolin-1 (Tyr 14): (A,B,C) and caveolin-1 (N-20): (D,E,F).
Application
Western blot analysis of caveolin-1 expression in non-transfected: (A) and human caveolin-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of caveolin-1 expression in non-transfected 293T: (A), mouse caveolin-1 transfected 293T: (B) and U-87 MG (C) whole cell lysates.
Application
Western blot analysis of caveolin-1 expression in non-transfected: (A) and human caveolin-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of caveolin-1 expression in non-transfected 293: (A), human caveolin-1 transfected 293: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of caveolin-1 expression in non-transfected control (A) and caveolin-1 siRNA transfected (B) HeLa cells. Blot probed with caveolin-1 (N-20): . Actin (I-19): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing membrane and cytoplasmic staining.
Application
Western blot analysis of caveolin-1 p22 expression in A-431 (A), NIH/3T3 (B), MDA-MB-231 (C) and SK-LMS-1 (D) whole cell lysates.| Application Notes | recommended for detection of α-caveolin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
