Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of NIK expression in non-transfected 293T: (A), human NIK transfected 293T: (B) and Cos (C) whole cell lysates.Application
Western blot analysis of NIK expression in non-transfected 293T: (A), human NIK transfected 293T: (B) and Cos (C) whole cell lysates.Application
Western blot analysis of NIK expression in control (A) and NIK-transfected (B) COS cells.| Product Name | NIK Antibody (H-248) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 700-947 mapping at the C-terminus of NIK (NFκB-inducing kinase) of human origin |
| Modification Notes | The NF˚B transcription factor can be activated by several cytokines including TNF and IL-1. The TNF receptor activates NF˚B through the TRAF2 adapter protein, whereas the IL-1 receptor activates NF˚B in a pathway involving TRAF6. Both TRAF2 and TRAF6 have been shown to interact with a Serine/Threonine kinase designated NF˚B inducing kinase (NIK), which appears to participate in the NF˚B signaling cascades triggered by both TNF and IL-1. NIK associates with, and is a costimulator for IkB kinase a (IKKå). IKKå, in turn, phosphorylates IkB, resulting in IkB degradation and NF˚B activation. NIK has sequence similarity to several kinases that participate in MAP kinase cascades. NIK appears to be uninvolved in the TRAF2-mediated activation of JNK by TNF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NIK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 700-947 mapping at the C-terminus of NIK (NF&kappa B-inducing kinase) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 700-947 mapping at the C-terminus of NIK (NFκB-inducing kinase) of human origin |
| Entrez Gene | 9020 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of NIK expression in non-transfected 293T: (A), human NIK transfected 293T: (B) and Cos (C) whole cell lysates.
Application
Western blot analysis of NIK expression in non-transfected 293T: (A), human NIK transfected 293T: (B) and Cos (C) whole cell lysates.
Application
Western blot analysis of NIK expression in control (A) and NIK-transfected (B) COS cells.| Application Notes | recommended for detection of NIK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
