Application
Western blot analysis of MSK2 expression in non-transfected: (A) and human MSK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MSK2 expression in A431 (A) and 293T (B) whole cell lysates.Application
Western blot analysis of MSK2 expression in non-transfected: (A) and mouse MSK2 transfected: (B) 293T whole cell lysates.| Product Name | MSK2 Antibody (A-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MSK2 of human origin by WB, IP, IF and IHC(P) |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. MSK2, also known as RPS6KA4 (ribosomal protein S6 kinase, 90kDa, polypeptide 4) or RSKB, is a 772 amino acid protein that localizes to the nucleus and contains one AGC-kinase C-terminal domain and two protein kinase domains. Using magnesium as a cofactor, MSK2 functions as a Ser/Thr kinase that is thought to play a role in the regulation of growth-factor and stress-induced transcriptional activation, specifically by catalyzing the ATP-dependent phosphorylation of target proteins. Multiple isoforms of MSK2 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MSK2 of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of MSK2 of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 8986 |
|---|
Application
Western blot analysis of MSK2 expression in non-transfected: (A) and human MSK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MSK2 expression in A431 (A) and 293T (B) whole cell lysates.
Application
Western blot analysis of MSK2 expression in non-transfected: (A) and mouse MSK2 transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
