Application
Western blot analysis of Cypher expression in L6 (A), IMR-32 (B) and Sol8 (C) whole cell lysates.| Product Name | Cypher Antibody (H-83) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 572-654 mapping within an internal region of Cypher of human origin |
| Modification Notes | Cypher, also known as LDB3 (LIM domain binding 3), ZASP (Z-band alternatively spliced PDZ-motif protein), ORACLE, PDLIM6 (PDZ and LIM domain 6), ldb3z1 or ldb3z4, is a 727 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains three LIM zinc-binding domains. Expressed primarily in skeletal muscle and at lower levels in brain, placenta and heart, Cypher is thought to function as an adaptor protein that, via its LIM domains, couples PKC-mediated signaling in striated muscle to the cytoskeleton. Defects in the gene encoding Cypher are associated with dilated cardiomyopathy 1C (CMD1C), dilated cardiomyopathy with left ventricular non-compaction and ZASP-related myofibrillar myopathy (MFM), all three of which are characterized by defects in cardiac muscle form and/or function. Six isoforms of Cypher exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cypher of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 572-654 mapping within an internal region of Cypher of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 572-654 mapping within an internal region of Cypher of human origin |
| Entrez Gene | 11155 |
|---|
Application
Western blot analysis of Cypher expression in L6 (A), IMR-32 (B) and Sol8 (C) whole cell lysates.| Application Notes | recommended for detection of Cypher of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
