Application
Western blot analysis of claudin-12 expression in T24 (A) and NTERA-2 cl.D1 (B) whole cell lysates and human recombinant claudin-12 fusion protein (C).Application
Western blot analysis of claudin-12 expression in mouse liver tissue extract.| Product Name | claudin-12 Antibody (H-49) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 196-244 mapping at the C-terminus of claudin-12 of human origin |
| Modification Notes | The claudin superfamily consists of many structurally related proteins that are important structural and functional components of tight junctions. Claudin-12, also known as CLDN12, is a 244 amino acid multi-pass membrane protein that is expressed in the brain, duodenum, jejunum, ileum, and colon. Claudin-12 localizes to cell junctions and may be involved in tight junction integrity by regulating hyperammonemia. Ammonia can alter brain capillary endothelial cell gene expression and transporter function. Claudin-12 is upregulated in enterocytes through vitamin D receptors which strongly suggest that claudin-12 forms paracellular Ca(2+) channels in intestinal epithelia and may be critical for vitamin D-dependent calcium homeostasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of claudin-12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 196-244 mapping at the C-terminus of claudin-12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 196-244 mapping at the C-terminus of claudin-12 of human origin |
| Entrez Gene | 9069 |
|---|
Application
Western blot analysis of claudin-12 expression in T24 (A) and NTERA-2 cl.D1 (B) whole cell lysates and human recombinant claudin-12 fusion protein (C).
Application
Western blot analysis of claudin-12 expression in mouse liver tissue extract.| Application Notes | recommended for detection of claudin-12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
