Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A-10 cells showing membrane localization.Application
Western blot analysis of A cyclase III expression in A-10 whole cell lysate.| Product Name | A cyclase III Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of A cyclase III of mouse origin |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, the dissociation of GTP-Ga s from Gbg and Ga s-mediated activation of adenylyl cyclase. Adenylyl cyclases of the type II family differ from other subforms in that they are conditionally stimulated by Ga s/bg subunits and regulated by PKC-mediated C-terminal phosphorylation. Both short- and long-term activation of D(2L) dopamine receptors result in a marked degree of sensitization of A cyclase I, II, V and IX, but not A cyclase VIII. The effects on A cyclase I, II and VIII is dependent upon the ability of these A cyclase isoforms to synergistically respond to selective activators in the presence of activated Ga s. Belonging to the adenylyl cyclase class IV family, A cyclase III is activated by Golf, which results in an elevation of cyclic AMP and subsequent activation of a cyclic nucleotide-gated channel. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of adenylyl cyclase III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of A cyclase III of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of A cyclase III of mouse origin |
| Entrez Gene | 109 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A-10 cells showing membrane localization.
Application
Western blot analysis of A cyclase III expression in A-10 whole cell lysate.| Application Notes | recommended for detection of adenylyl cyclase III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
