Application
Western blot analysis of Topo IIα expression in Jurkat nuclear extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include Topo IIα (H-231): (A,B) and p-Topo IIα (Thr 1343): (C,D).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.Application
Western blot analysis of Topo IIα expression in K-562 nuclear extract.| Product Name | Topo IIα Antibody (H-231) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1301-1531 mapping at the C-terminus of Topo IIα of human origin |
| Modification Notes | DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIå and Topo II∫. Topo II∫ and Topo IIå, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DNA Topoisomerase IIα of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1301-1531 mapping at the C-terminus of Topo II&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1301-1531 mapping at the C-terminus of Topo IIα of human origin |
| Entrez Gene | 7153 |
|---|
Application
Western blot analysis of Topo IIα expression in Jurkat nuclear extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include Topo IIα (H-231): (A,B) and p-Topo IIα (Thr 1343): (C,D).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
Application
Western blot analysis of Topo IIα expression in K-562 nuclear extract.| Application Notes | recommended for detection of DNA Topoisomerase IIα of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
