Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.Application
Western blot analysis of HDAC2 expression in HeLa whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.Application
Western blot analysis of HDAC2 expression in K-562 (A), phorbol-induced Jurkat (B) and NIH/3T3 (C) nuclear extracts and mouse testis extract (D).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.| Product Name | HDAC2 Antibody (H-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 435-488 mapping at the C-terminus of HDAC2 of human origin |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 435-488 mapping at the C-terminus of HDAC2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 435-488 mapping at the C-terminus of HDAC2 of human origin |
| Entrez Gene | 3066 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear and cytoplasmic staining of neuronal cells.
Application
Western blot analysis of HDAC2 expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.
Application
Western blot analysis of HDAC2 expression in K-562 (A), phorbol-induced Jurkat (B) and NIH/3T3 (C) nuclear extracts and mouse testis extract (D).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.| Application Notes | recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
