Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GKLF expression in non-transfected 293T: (A), mouse GKLF transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of GKLF expression in HeLa nuclear extract.| Product Name | GKLF Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GKLF of human origin |
| Modification Notes | The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation (1-4). The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene (5). Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF (6-8). EKLF is expressed principally in erythroid tissues (6), and LKLF expression is limited to the lung (7). GKLF is found predominantly in gut and has been shown to be expressed during growth arrest (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of GKLF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GKLF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GKLF of human origin |
| Entrez Gene | 9314 |
|---|
Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of GKLF expression in non-transfected: (A) and human GKLF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GKLF expression in non-transfected 293T: (A), mouse GKLF transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of GKLF expression in HeLa nuclear extract.| Application Notes | recommended for detection of GKLF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
