Application
Western blot analysis of FACE-1 expression in mouse cerebellum tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane localization.| Product Name | FACE-1 Antibody (U-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of FACE-1 of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Modification Notes | FACE-1, or Zinc metalloproteinase Ste24 homolog (Zmpste24), is a metalloproteinase responsible for cleaving prelamin A to lamin A, a component of the nuclear envelope. An integral membrane protein, FACE-1 is widely expressed in tissues throughout the human body and uses zinc as a cofactor, with one zinc ion per subunit. Mutations in the FACE-1 gene are linked to laminopathies in humans, including restrictive dermopathy (RD) and mandibuloacral dysplasia (MAD), both characterized by severe developmental abnormalities and in the case of RD, early neonatal death. Accumulation of unprocessed prelamin A may be responsible for deficits associated with these genetic disorders. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of FACE-1 of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of FACE-1 of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 10269 |
|---|
Application
Western blot analysis of FACE-1 expression in mouse cerebellum tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
