Application
Western blot analysis of p130 expression in mouse testis tissue extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.Application
Western blot analysis of p130 expression in untreated (A) and phorbol ester-treated (B) Jurkat whole cell lysates and C32 nuclear extract (C).Application
Western blot analysis of Rb-related p130 expression in Jurkat (A) and C32 (B) nuclear extracts.| Product Name | p130 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of p130 of human origin |
| Modification Notes | The human retinoblastoma gene product Rb plays an important role in the negative regulation of cell proliferation. The Rb family includes p107 and p130, which form complexes with E2F proteins, and share a high degree of structural homology in the adenovirus E1A binding domain (i.e., “pocket region”), which plays a primary role in the function of these proteins. The Rb family members undergo cell cycle dependent phosphorylation during mid-G1 to S phase transition, which is dependent upon the activity of cyclin D/cdk4. In contrast to pRb and p107, p130 is also phosphorylated during G0 and the early G1 phase of the cell cycle. p130 is specifically phosphorylated on serine and threonine residues in cells arrested in G0 by serum deprivation or density arrest, and these residues are clustered within a short co-linear region unique to p130 defined as the Loop. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of p130 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of p130 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of p130 of human origin |
| Entrez Gene | 5934 |
|---|
Application
Western blot analysis of p130 expression in mouse testis tissue extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
Application
Western blot analysis of p130 expression in untreated (A) and phorbol ester-treated (B) Jurkat whole cell lysates and C32 nuclear extract (C).
Application
Western blot analysis of Rb-related p130 expression in Jurkat (A) and C32 (B) nuclear extracts.| Application Notes | recommended for detection of p130 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
