Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing cytoplasmic localization.Application
Western blot analysis of ERRγ expression in Jurkat whole cell lysate.| Product Name | ERRγ Antibody (Z-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ERRγ of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Estrogen and progesterone receptors are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements (EREs) alters transcription of ERE-containing genes. Estrogen receptor-related proteins (ERRå, ∫ and ©) are orphan nuclear receptors. Like estrogen receptors, ERRs bind specifically to EREs to activate reporter genes. EREs are constitutively active without binding to estrogen. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B), which arise from alternative splicing. In most cells, PR-B functions as a transcriptional activator of progesterone-responsive genes, whereas PR-A functions as a transcriptional inhibitor of all steroid hormone receptors. mPR is a membrane progestin receptor. The predicted 436-amino acid ERR© protein which presumably localizes to the nucleus, is expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ERR&gamma of mouse rat human and zebrafish origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of ERRγ of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 2104 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing cytoplasmic localization.
Application
Western blot analysis of ERRγ expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
