Application
Western blot analysis of CNOT3 expression in non-transfected: (A) and human CNOT3 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of CNOT3 expression in Jurkat whole cell lysate.Application
Western blot analysis of CNOT3 expression in K-562 whole cell lysate.| Product Name | CNOT3 Antibody (D-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CNOT3 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | CNOT3 is a widely expressed subunit of the CCR4-NOT transcription complex and belongs to the CNOT2/3/5 family. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. Similar to CNOT2, CNOT3 contains the specialized protein motif NOT-Box. This conserved motif confers a transcription repression function to CNOT3. Repression by the NOT-Box is sensitive to treatment with the histone deacetylase (HDAC) inhibitor trichostatin A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CNOT3 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of CNOT3 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 4849 |
|---|
Application
Western blot analysis of CNOT3 expression in non-transfected: (A) and human CNOT3 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of CNOT3 expression in Jurkat whole cell lysate.
Application
Western blot analysis of CNOT3 expression in K-562 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
