Application
Western blot analysis of α1-chimaerin expression in non-transfected: (A) and human α1-chimaerin transfected: (B) 293 whole cell lysates.| Product Name | α-chimaerin Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 144-183 mapping within an internal region of α2-chimaerin of human origin |
| Modification Notes | The Rac-GAP chimaerin family member å-chimaerin (also known as N-chimaerin or rho GTPase-activating protein 2) has two splice variants: å1 and å2. The å1-chimaerin variant is a neuron-specific, diacylglycerol-binding and GTPase-activating protein for ras-related protein Rac 1. This variant lacks the N-terminal SH2 domain that is present in the å2 variant. By inactivating Rac 1, å1-chimaerin plays a significant role in the regulation of dendritic growth during neuronal development. It is recruited to the plasma membrane by phospholipase C∫-coupled cell surface receptors activating the downstream generation of DAG (diacylglycerol). Overexpression of å1-chimaerin results in dendritic spine retraction and the loss of dendritic branches. In the presence of reduced neuronal activity, å1-chimaerin expression is down-regulated resulting in an increase in spine growth and dendritic branching. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of α1-chimaerin and α2-chimaerin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 144-183 mapping within an internal region of &alpha 2-chimaerin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 144-183 mapping within an internal region of α2-chimaerin of human origin |
| Entrez Gene | 1123 |
|---|
Application
Western blot analysis of α1-chimaerin expression in non-transfected: (A) and human α1-chimaerin transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of α1-chimaerin and α2-chimaerin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
