| Product Name | granuphilin Antibody (H-255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 44-298 mapping near the N-terminus of granuphilin of human origin |
| Modification Notes | Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic b-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal insulin secretion but also inhibits high K+-induced insulin secretion. The effect of granuphilin on insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of granuphilin of mouse, rat, equine, bovine, porcine and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 44-298 mapping near the N-terminus of granuphilin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 44-298 mapping near the N-terminus of granuphilin of human origin |
| Entrez Gene | 94121 |
|---|
| Application Notes | recommended for detection of granuphilin of mouse, rat, equine, bovine, porcine and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
