Application
Western blot analysis of PI 3-kinase p101 expression in K-562 whole cell lysate.| Product Name | PI 3-kinase p101 Antibody (I-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PI 3-kinase p101 of human origin by WB, IP and ELISA |
| Modification Notes | PI 3-kinase p101 is an 880 amino acid protein that acts as a regulatory subunit of the PI3 kinase γ complex. PI 3-kinase p101 interacts with PI 3-kinase p110γ to form the PI3 kinase γ complex, which is activated by G∫γ proteins and plays a role in many physiological processes, such as cardiac function, neutrophil chemotaxis and mast cell degranulation. Specifically, the PI3 kinase γ complex is involved in suppression of apoptosis, cellular transport and cell motility. Binding of the PI 3-kinase p110γ subunit to PI 3-kinase p101 is dependent on the N-terminal region of PI 3-kinase p101. With highest expression in leukocytes, spleen lymph node thymus and bone marrow, PI 3-kinase p101 is subcellularly located in the nucleus, cytoplasm, or it can exist as a peripheral membrane protein. There are two isoforms of PI 3-kinase p101 that are produced as a result of alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PI 3-kinase p101 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PI 3-kinase p101 of human origin by WB, IP and ELISA |
| Entrez Gene | 23533 |
|---|
Application
Western blot analysis of PI 3-kinase p101 expression in K-562 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
