| Product Name | p-Ras-GRF1 Antibody (Ser 929) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 929 phosphorylated Ras-GRF1 of human origin by WB, IP and ELISA |
| Modification | Phosphoralated |
| Modification Notes | A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP bound state. To reach their active GTP bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 (also known as Ras-GRF1 p140). Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP binding proteins. Of interest, a region mapping within the amino-terminal domain of Ras-GRF1 is similar to both the human breakpoint cluster protein Bcr and the Dbl proto-oncogene product, a guanine nucleotide-releasing factor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2 p135 is highly homologous to Ras-GRF1 and the two proteins appear to function in a similar manner. Human Ras-GRF1 is subject to phosphorylation at specific amino acid residues, including Ser 929. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 929 phosphorylated Ras-GRF1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 929 phosphorylated Ras-GRF1 of human origin by WB, IP and ELISA |
| Entrez Gene | 5923 |
|---|
| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
