Application
Western blot analysis of PIP1 expression in K-562 (A), SK-N-MC (B) and Hep G2 (C) nuclear extracts.| Product Name | PIP1 Antibody (A-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping to N-terminal and C-terminal peptides of PIP1 of human origin |
| Modification Notes | PAK1-interacting protein 1 (PIP1), also known as p21-activated protein kinase-interacting protein 1 or WDR repeat-containing protein 84, is a 392 amino acid nuclear protein. PIP1 acts as a negative regulator of åPAK by binding to the N-terminal regulatory domain of åPAK. This interaction results in an inactive homodimer conformation of åPAK, which inhibits the binding of small, activating GTPases. Overexpression of PIP1 causes abnormally rounded cell morphology and reorganized actin stress fibers in embryonic fibroblasts. Containing five WD repeat domains, PIP1 is expressed in the heart, liver, lung, kidney, muscle, brain, small intestine, colon and thymus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of PIP1 of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping to N-terminal and C-terminal peptides of PIP1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping to N-terminal and C-terminal peptides of PIP1 of human origin |
| Entrez Gene | 55003 |
|---|
Application
Western blot analysis of PIP1 expression in K-562 (A), SK-N-MC (B) and Hep G2 (C) nuclear extracts.| Application Notes | recommended for detection of PIP1 of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
