Application
Western blot analysis of USE1 expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic staining.| Product Name | USE1 Antibody (B-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against USE1 of human origin |
| Modification Notes | In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and, after covalent modification, delivers them to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). Unconventional SNARE in the ER 1, also known as USE1 or protein p31, is a 259 amino acid t-SNARE that forms a larger complex with ZW10, RINT-1 and Syntaxin 18. Upon Mg2+-AP treatment in the presence of NSF and å-SNAP, ZW10, RINT-1 and USE1 dissociate from Syntaxin 18. USE1 is a single-pass type IV membrane protein that is localized to the endoplasmic reticulum membrane. Three named isoforms exist for USE1 as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of USE1 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against USE1 of human origin |
|---|---|
| Tissue Specificity | raised against USE1 of human origin |
| Entrez Gene | 55850 |
|---|
Application
Western blot analysis of USE1 expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of USE1 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
