Application
Western blot analysis of SAP 155 expression in non-transfected: (A) and mouse SAP 155 transfected: (B) 293T whole cell lysates and Jurkat (C), K-562 (D), Ramos (E) and MCF7 (F) nuclear extracts.Application
Western blot analysis of SAP 155 expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Product Name | SAP 155 Antibody (P-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against SAP 155 of human origin |
| Modification Notes | SAP 155 (spliceosome-associated protein 155), also known as SF3B1, SF3B155 (splicing factor 3b, subunit 1, 155 kDa), PRP10 or PRPF10, is a 1,304 amino acid member of the SF3B1 family and contains eleven HEAT repeats. Localized to nuclear speckles and also to the cytoplasm during mitosis, SAP 155 is a subunit of the SF3B splicing factor. The SF3B splicing factor is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130, SAP 145 and SAP 155. Concomitant with splicing catalysis, SAP 155 is phosphorylated at its N-terminal Thr-Pro dipeptide motifs by Dyrk1A and cyclin E/Cdk2. This modification of SAP 155 is vital for a functional spliceosome as it is an essential event in the basic splicing reaction. Due to alternative splicing events, various SAP 155 isoforms are produced. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SAP 155 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against SAP 155 of human origin |
|---|---|
| Tissue Specificity | raised against SAP 155 of human origin |
| Entrez Gene | 23451 |
|---|
Application
Western blot analysis of SAP 155 expression in non-transfected: (A) and mouse SAP 155 transfected: (B) 293T whole cell lysates and Jurkat (C), K-562 (D), Ramos (E) and MCF7 (F) nuclear extracts.
Application
Western blot analysis of SAP 155 expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of SAP 155 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
