Application
Western blot analysis of FKHR phosphorylation in non-transfected: (A) and mouse FKHR transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed EGF and serum-treated HeLa cells showing nuclear localization.Application
Blots were probed with p-FKHR (Ser 256): preincubated with cognate phosphorylated peptide (A) and p-FKHR (Ser 256): (B,C,D).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-FKHR Antibody (Ser 256) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 256 of FKHR of human origin |
| Modification | Phosphoralated |
| Modification Notes | The transcription factor forkhead in rhabdomyosarcoma (FKHR), which is inhibited by insulin and IGF-1, enhances transcription. FKHR has been implicated in alveolar rhabdomyosarcoma, a soft tissue tumor wherein a chromosomal translocation [t(2;12)(q35;q14)] occurs between the FKHR and PAX3 genes, resulting in a novel chimeric protein with abnormal levels of expression. FKHR becomes phosphorylated at Ser 319, Ser 256 and Thr 256 by Protein kinase B (PKB) in a phosphoinsoditide 3-(PI3) kinase/Akt dependent pathway, resulting in the inactivation and subsequent nuclear exit of FKHR. In addition, FKHR becomes phosphorylated at Ser 329, also resulting in decreased FKHR activity and diminished nuclear FKHR concentration. However, phosphorylation of FKHR at Ser 329 is not mediated by a PI3-kinase-dependent pathway, but by an alternate mechanism. Dual-specificity tyrosine-phosphorylated and regulated kinase 1A (DYRK1A), which co-localizes to the same region of the nucleus as FKHR, specifically phosphorylates FKHR at Ser 329 in rabbit skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 256 phosphorylated FKHR of human origin, correspondingly phosphorylated Ser 253 of mouse origin and correspondingly phosphorylated Ser 250 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 256 of FKHR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 256 of FKHR of human origin |
| Entrez Gene | 2308 |
|---|
Application
Western blot analysis of FKHR phosphorylation in non-transfected: (A) and mouse FKHR transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed EGF and serum-treated HeLa cells showing nuclear localization.
Application
Blots were probed with p-FKHR (Ser 256): preincubated with cognate phosphorylated peptide (A) and p-FKHR (Ser 256): (B,C,D).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 256 phosphorylated FKHR of human origin, correspondingly phosphorylated Ser 253 of mouse origin and correspondingly phosphorylated Ser 250 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
