Application
Antibodies tested include p-MEK-1 (Thr 291): (A,B,C) and MEK-1 (H-8): (D,E,F).Application
Antibodies tested include p-MEK-1 (Thr 291): (A, B) and MEK-1 (C-18): (C, D).Application
Western blot analysis of MEK-1 phosphorylation in EGF treated (A) and EGF and lambda protein phosphatase treated (B) 293T whole cell lysates.Application
Western blot analysis of phosphorylated MEK-1 expression in untreated (A) and serum-treated (B) 293 whole cell lysates.| Product Name | p-MEK-1 Antibody (Thr 291) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 291 of MEK-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAFor MEKK1) positively regulates the kinase activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Thr 291 phosphorylated MEK-1 of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 291 of MEK-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 291 of MEK-1 of human origin |
| Entrez Gene | 5604 |
|---|
Application
Antibodies tested include p-MEK-1 (Thr 291): (A,B,C) and MEK-1 (H-8): (D,E,F).
Application
Antibodies tested include p-MEK-1 (Thr 291): (A, B) and MEK-1 (C-18): (C, D).
Application
Western blot analysis of MEK-1 phosphorylation in EGF treated (A) and EGF and lambda protein phosphatase treated (B) 293T whole cell lysates.
Application
Western blot analysis of phosphorylated MEK-1 expression in untreated (A) and serum-treated (B) 293 whole cell lysates.| Application Notes | recommended for detection of Thr 291 phosphorylated MEK-1 of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
