Application
Western blot analysis of phosphorylated AMPKα1 expression in untreated (A) and heat shock-treated (B) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic localization.| Product Name | p-AMPKα1 Antibody (Ser 496) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 496 of AMPKα1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic å subunit and regulatory ∫ and © subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKå1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKå1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 496 phosphorylated AMPKα1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 496 of AMPK&alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 496 of AMPKα1 of human origin |
| Entrez Gene | 5563 |
|---|
Application
Western blot analysis of phosphorylated AMPKα1 expression in untreated (A) and heat shock-treated (B) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Ser 496 phosphorylated AMPKα1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
