Application
Western blot analysis of TRC8 expression in non-transfected 293T: (A), human TRC8 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | TRC8 Antibody (H-89) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 576-664 mapping at the C-terminus of TRC8 of human origin |
| Modification Notes | TRC8 (translocation in renal carcinoma on chromosome 8), also known as RNF139 (ring finger protein 139), RCA1 or HRCA1 (hereditary renal cancer associated 1), is a multi-pass membrane protein that is predominantly expressed in testis, adrenal gland and placenta and is expressed at lower levels in liver, skeletal muscle, pancreas, kidney, brain, heart and lung. Localizing to the endoplasmic reticulum (ER), TRC8 contains ten transmembrane segments, a sterol-sensing domain and one RING-type zinc finger and may function as a ubiquitin ligase and signaling receptor. TRC8 physically interacts with VHL (Von Hippel Lindau disease tumor suppressor), and the inhibition of either of these proteins leads to the same ventral midline defect. Disruption of the TRC8 gene, caused by the 3;8 chromosomal translocation, is associated with hereditary renal cell carcinoma (RCC), suggesting that TRC8 is a potential tumor suppressor for RCC. Further supporting its role as a tumor suppressor, TRC8 mediates the induction of G2/M phase arrest, increased apoptosis and decreased DNA synthesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of translocation in renal carcinoma on chromosome 8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 576-664 mapping at the C-terminus of TRC8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 576-664 mapping at the C-terminus of TRC8 of human origin |
| Entrez Gene | 11236 |
|---|
Application
Western blot analysis of TRC8 expression in non-transfected 293T: (A), human TRC8 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of translocation in renal carcinoma on chromosome 8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
