Application
Western blot analysis of Deltex-3 expression in mouse embryonic brain (A) and mouse brain (B) tissue extracts and SK-N-MC (C), F9 (D) and NTERA-2 cl.D1 (E) whole cell lysates.Application
Western blot analysis of Deltex-3 expression in non-transfected: (A) and mouse Deltex-3 transfected: (B) 293T whole cell lysates.| Product Name | Deltex-3 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 mapping at the N-terminus of Deltex-3 of human origin |
| Modification Notes | The Deltex family of proteins (Deltex-1, 2, 3 and 4) are mammalian homologs of Drosophila Deltex. This family contains two WWE domains and a C-terminal RING-finger domain, which are regions that are frequently found in E3 ubiquitin ligases. Deltex-3, also known as RNF154 (RING finger protein 154), is a 347 amino acid cytoplasmic protein that acts as both a negative and positive regulator of Notch, depending on the developmental and cell context. Though primarily acting as a homomultimer, Deltex-3 may form a heteromultimer with other Deltex proteins. Like other Deltex family members, Deltex-3 functions as a ubiquitin E3 ligase that shows highest activity in conjunction with the E2 enzyme UBE2D. There are two isoforms of Deltex-3 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Deltex-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 mapping at the N-terminus of Deltex-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 mapping at the N-terminus of Deltex-3 of human origin |
Application
Western blot analysis of Deltex-3 expression in mouse embryonic brain (A) and mouse brain (B) tissue extracts and SK-N-MC (C), F9 (D) and NTERA-2 cl.D1 (E) whole cell lysates.
Application
Western blot analysis of Deltex-3 expression in non-transfected: (A) and mouse Deltex-3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Deltex-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
