Application
Western blot analysis of Rho GDI expression in HL-60 (A), HeLa (B) and SK-BR-3 (C) whole cell lysates.| Product Name | Rho GDI Antibody (FL-204) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus Rho GDIα of human origin |
| Modification Notes | The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTP-bound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIå, Ly-GDI (also known as Rho GDI∫ and previously known as GDI/D4) and Rho GDI©. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rho GDIα, Ly-GDI (Rho GDIβ) and Rho GDIγ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus Rho GDI&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus Rho GDIα of human origin |
| Entrez Gene | 396 |
|---|
Application
Western blot analysis of Rho GDI expression in HL-60 (A), HeLa (B) and SK-BR-3 (C) whole cell lysates.| Application Notes | recommended for detection of Rho GDIα, Ly-GDI (Rho GDIβ) and Rho GDIγ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
